Catalog Number - 68508-15P-2-3M

Cogent RP C8™ Wide Pore HPLC Columns are designed for reversed-phase separations of larger molecules that require enhanced pore accessibility and efficient mass transfer. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity silica and is fully double end capped to minimize secondary interactions while providing reproducible retention, excellent peak shape, and long column life. The 300 Å wide pore structure allows proteins, peptides, and other higher molecular weight compounds to access more of the bonded surface area, making this phase particularly suitable for biomolecule analyses and challenging large-molecule separations.

68508-15P-2-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 150 mm length format. The 150 mm column length provides maximum resolving power and peak capacity for complex separations while maintaining the low solvent consumption advantages of a narrow-bore column. The wide pore architecture improves accessibility and mass transfer for proteins, peptides, and long-chain fatty acids that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for hydrophobic and moderately non-polar compounds.

This column is frequently selected for LC-MS applications when the highest analytical resolution is required for biomolecules and other large analytes. The 2.1 mm ID format minimizes mobile phase consumption and is highly compatible with mass spectrometric detection. Optimum performance is typically achieved at approximately 0.2-0.3 mL/min. Standard HPLC systems used with this column must be capable of delivering accurate and reproducible low flow rates, and low-volume detector flow cells are recommended to minimize extra-column dispersion and maintain chromatographic efficiency.

Key Benefits

• RP C8™ Wide Pore stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 300 Å wide pore design

• Ideal for proteins and peptides

• High resolving power

• Lower retention than C18 phases

• Reduced solvent consumption

• Excellent for LC-MS applications

Typical Applications

• LC-MS analysis

• Reversed-phase HPLC

• Protein analysis

• Peptide analysis

• Biomolecule separations

• Long-chain fatty acid analysis

• Pharmaceutical analysis

• Biopharmaceutical analysis

• Method development

• High-resolution analytical separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Recommended Flow Rate: 0.2-0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed for large molecule reversed-phase separations

• 300 Å pores enhance accessibility for proteins, peptides, and biomolecules

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for peptide analysis, protein analysis, and long-chain fatty acid separations

• 150 mm length provides maximum resolving power and peak capacity

• 5 µm particles provide low backpressure and broad instrument compatibility

• Requires an HPLC system capable of precise and reliable low flow rate delivery

• Low-volume detector flow cells are recommended to maximize chromatographic efficiency

• Particularly well suited for LC-MS workflows involving biomolecules